Table 1. Summary of experimental efficiencies to generate the genetic balancers IV.
| Balancer name | Cas9 targets | Targeting vector | Distance (cM) | Background genotype | P0 wormsa | F1 wormsb | Phenotype in F2c | F1 PCRd | F2 PCRe | Ratio (%)f |
|---|---|---|---|---|---|---|---|---|---|---|
| tmIn42-44 | egl-4 unc-17 | − | 11.5 | WT (N2) | 146 | 900 | 163 | 4 | 3 | 0.33 |
| tmIn45 | egl-4 unc-17 | − | 11.5 | lig-4 (tm750) | 149 | 723 | 87 | 10 | 1 | 0.13 |
| − | egl-4 unc-17 | + | 11.5 | WT (N2) | 107 | 755 | 102 | 12 | 0 | 0 |
| tmIn1 | egl-4 unc-17 | + | 11.5 | lig-4 (tm750) | 31 | 136 | 40 | 6 | 1 | 0.73 |
| tmIn2 | ced-2 unc-17 | + | 13.5 | lig-4 (tm750) | 48 | 168 | 24 | 2 | 1 | 0.60 |
| tmIn3 | jtr-1 unc-17 | + | 16.4 | lig-4 (tm750) | 46 | 312 | 96 | 25 | 1 | 0.32 |
| − | csn-4 egl-4 | + | 7.6 | lig-4 (tm750) | 39 | 168 | 64 | 4 | 0 | 0 |
aTotal number of injected P0 worms.
bTotal number of fluorescent F1 worms obtained.
cNumber of F1 strains whose progeny showed phenotypes.
dNumber of F1 strains that showed rearrangement-specific PCR bands in the first screening.
eNumber of F2 strains that showed rearrangement-specific PCR bands in the second screening.
fIsolated genetic balancer/total number of fluorescent F1 worms.