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. 2016 Mar 8;10(10):2498–2513. doi: 10.1038/ismej.2016.30

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Copyright © 2016 International Society for Microbial Ecology

This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/4.0/

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(a) Localization of transposon (Tn) mutants in the four genes of the rhamnose operon located on the 65-kb plasmid of Phaeobacter inhibens DSM 17395 (rmlC, rmlB, rmlD and rmlA). (b) Box plot of biofilm formation of the rhamnose transposon mutants monitored by crystal violet assays of three biological and eight technical replicates. The P. inhibens wild type (wt) and the transposon mutant Tn_4121 (inserted in the non-coding region between PGA1_c16400 and PGA1_c16410; position 1 701 875; plus strand) served as a positive control (PC). Mean OD₆₀₀ of MB medium (negative control (NC); 0.100), wt (0.877), PC (Tn_4121; 0.888), ΔrmlC (0.409), Δ rmlB (0.402), Δ rmlD (0.410), Δ rmlA (0.391) and Δ65 kb (0.128). (c) Motility assay on 0.3% agar for the detection of swimming motility. Plates were incubated for 3 days at 28 °C. ΔC, ΔB, ΔD and ΔA, Tn-mutants of the rhamnose operon; Δ65, P. inhibens DSM 17395 curing mutant lacking the 65-kb plasmid.