Table 3. Assembly statistics of the six genome bins containing scaffolds to which proteins showing incorporation of 13C mapped to in the protein-SIP analysis.
| Phylogenetic affiliation | No. of contigs | Total length (bp) | GC (%) | N50 | Foulum coverage | Lynggaard coverage | No. essential unique genes | No. of total essential genes | Figure 4a |
|---|---|---|---|---|---|---|---|---|---|
| Clostridia (Gelria) | 128 | 2 092 416 | 59.40 | 25 636 | 731.79 | 593.5 | 104 | 107 | Gelria (C1) |
| Clostridia (Gelria) | 306 | 1 833 135 | 49.50 | 11 477 | 405.00 | 71.02 | 94 | 95 | Gelria (C2) |
| Clostridia | 145 | 1 786 211 | 56.90 | 19 622 | 140.58 | 12.09 | 100 | 103 | Clostridia (C3) |
| Clostridia | 71 | 2 167 847 | 53.80 | 71 792 | 125.47 | 4.93 | 102 | 104 | Clostridia (C4) |
| Clostridia | 59 | 2 188 512 | 53.60 | 61 047 | 102.11 | 0.54 | 100 | 103 | Clostridia (C5) |
| Methanomicrobia (Methanoculleus) | 161 | 2 200 216 | 59.40 | 24 804 | 148.67 | 1.09 | 33 | 35 | Methanoculleus (M) |
The number of essential genes was evaluated using 107 Hidden Markov models of protein coding-essential single-copy genes.