Comparison of the genes flanking the alcohol (alc) and aldehyde (ald) dehydrogenase genes (red) in Methanosphaera sp. WGK6, Methanobrevibacter sp. AbM4, Methanobrevibacter boviskoreani JH1, Methanobrevibacter wolinii SH and Methanobrevibacter olleyae DSMZ 16632 (a–e, respectively). A high degree of gene conservation is seen among strains AbM4, JH1 and SH, each possessing a coenzyme F420-reducing hydrogenase complex upstream (frhADGB—purple); and a transposase (black), F420:NADP oxidoreductase (fno—orange) and methionine aminopeptidase (map—blue) located downstream, with strains AbM4 and JH1 also possessing an aspartate dehydrogenase (asd—brown). Methanobrevibacter olleyae DSMZ 16632 is surrounded by hypothetical proteins (hyp—gray); however, there is a transposase (black) downstream of the dehydrogenase genes, while strain WGK6 is flanked by an membrane protein (omp—green), hypothetical proteins (hyp—gray) and a putative histone (yellow).