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. Author manuscript; available in PMC: 2016 Sep 21.
Published in final edited form as: J Immunol. 2015 Aug 7;195(6):2540–2551. doi: 10.4049/jimmunol.1501019

Figure 2. LC-MS fractionation of Listeria polar lipids.

Figure 2

A. Listeria polar lipids were subjected to LC-MS fractionation by head group. B. Inset from panel A, highlighting the light scattering peaks collected for LC-MS fractions A–F. C–E. 14S.6 (left) and TBA7 (right) hybridomas were incubated with LC-MS fractions A or B (panel C) fractions C or D (panel D) or with fractions E or F (Panel E) in the presence of RAW (grey lines) or RAW-CD1d (black lines) cells in triplicate. Hybridomas were also co-cultured with RAW or RAW-CD1d cells without adding exogenous lipid in duplicate (gray and black asterisks). After 16–18 h of co-culture, supernatants were assayed for the presence of IL-2 by ELISA. Each graph is representative of three independent experiments and presented as mean ± SEM.