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. 2016 Jul 7;21(9):912–922. doi: 10.1177/1087057116657269

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© 2016 Society for Laboratory Automation and Screening

This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 3.0 License (http://www.creativecommons.org/licenses/by-nc/3.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access page (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Figure 2. — c-Met–dependent phenotypic changes. (A) Inhibition of hepatocyte growth factor (HGF)–induced invasion in 3D-cultured PC-3 cells by function blocking c-Met Fab antibody 5D5 (Genentech, South San Francisco, CA) and stimulation of invasion by the crosslinking and activating bivalent IgG isoform. Principal components analysis (PCA) trained on unstimulated and stimulated control, PC0 shown and scaled as Z score to unstimulated control. Results are shown as means of quadruplicate wells and standard deviations. (B) 5D5 Fab (triangles) causes a shift in phenotype from stimulated (empty circles) back to unstimulated control (filled circles). Point size of markers for Fab treatment increases with concentration (seven concentrations; range, 0.316–316 nM). Individual data points are shown as a 3D scatterplot with PC0, PC1, and PC2.