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. 2016 Sep 21;7:1448. doi: 10.3389/fmicb.2016.01448

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Copyright © 2016 Klug, Santigli, Westendorf, Tangl, Wimmer and Grube.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Experimental outline. (A) Dental splint with six human enamel-dentin slabs (white squares, a, top view; b, side view) fixed in the upper jaw (c, front view; d, side view). (B) Sketch of the biofilm reactor setup (from left to right): Supply bottle filled with BHI medium (orange), peristaltic pump, bubble trap, DFR 110 biofilm reactor on heating plate (34°C), arrows indicate medium flow direction. (C) Top view sketch of the enamel-dentin slab distribution in biofilm reactor chambers. (D) Timeline and slab use (orange, live/dead staining; blue, pyrosequencing).