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. 2016 Sep 19;213(10):1973–1981. doi: 10.1084/jem.20151065

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© 2016 Drennan et al.

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Figure 2. — iNKT cell development is regulated by A20. (A and B) Frequency and absolute iNKT cell counts in thymus (Thy), blood (Bld), spleen (Spl), liver (Lvr), lung (Lng), and PLN of control (n = 8) and CD4-cre;A20^F/F (n = 9) mice, respectively. Data are from four independent experiments pooled. (C and D) Frequency and absolute cell counts of ST0, ST1, ST2, and ST3 iNKT cells isolated from thymi of control (n = 8) and CD4-cre;A20^F/F (n = 9) mice, respectively. Data are pooled from two independent experiments. (E) Flow cytometric analysis and absolute cell counts (F) of NKT1^a, NKT1^b, NKT2, and NKT17 sublineages present in thymus, blood, spleen, liver, lung, and PLN of control (n = 8) and CD4-cre;A20^F/F (n = 9) mice, respectively. Data are from four independent experiments pooled. *, P < 0.05; **, P < 0.005; ***, P < 0.0005.