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. 2016 Sep 19;213(10):2019–2037. doi: 10.1084/jem.20160157

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© 2016 Bi et al.

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Figure 4. — Notch inhibitor DAPT inhibits cell proliferation of human DDLPS cells. (A) Representative IHC staining results of N1ICD and HES1 in human adipose tissue and LPS sections (n = 4/group). Arrows point to positive cells. (B) Crystal violet staining result of DMSO or Notch inhibitor DAPT-treated human DDLPS cell line LPS246. (C) Quantification of densitometry in B. n = 3. (D) Relative expression levels of Notch target genes in LPS246 cells. n = 8. (E) Immunofluorescent images of DMSO or DAPT-treated LPS246 cells. n = 3. (F) Quantification of Ki67⁺ percentages of cells as in E. n = 3. Bars, 50 µm. *, P < 0.05. Bar graphs indicate mean ± SEM.