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. 2016 Sep 19;213(10):1951–1959. doi: 10.1084/jem.20160486

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© 2016 Kim et al.

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Figure 2. — CD226⁺ macrophages develop after birth. (A and B) Peritoneal cells after pregating CD11b⁺ CD115⁺ macrophages (A) and the MHC II⁺ macrophage subpopulation (B) from P2, P4, P6, P8, or P12 in CD11c-EYFP mice. (C) Quantification of total cell, F4/80⁺ICAM2⁺, MHC II^lo, or MHC II⁺ macrophage subpopulations shown in A and B. P-values from multiple comparisons of two-way ANOVA: *, P < 0.05; **, P < 0.01; ****, P < 0.0001. Mean ± SEM. (D) Ly6C and GFP expression on MHC II⁺ and MHC II^lo cells from P4 in CX₃CR1^gfp/+ mice. Data are the representative of two independent experiments (n = 3 mice/experiment). (E) EYFP expression by MHC II^lo cells, CD226⁺ macrophages, and MHC II⁺ CD226⁻ macrophages in CD11c-EYFP mice. (A–C and E) Data are pooled from at least four independent experiments (P0–2, n = 8 mice; P4–6, n = 8 mice; P8–10, n = 4 mice; P12–14, n = 4 mice).