Figure 4.

IRF4 governs the development of CD226⁺ macrophages. (A) Flow cytometric analysis of F4/80⁺ ICAM2⁺ and MHC II⁺ macrophage subpopulations in IRF4^+/+ and IRF4^−/− mice. (B–D) Quantification of peritoneal F4/80⁺ macrophages and MHC II⁺ macrophages (B), MHC II⁺ macrophage subpopulations (C), and blood monocytes (D) in IRF4^+/+ and IRF4^−/− mice. Data are from two independent experiments (n = 5–7, mean ± SEM). P-values, unpaired Student’s t test: **, P < 0.01. (E) Flow cytometric analysis of F4/80⁺ ICAM2⁺ macrophages and MHC II⁺ macrophage subpopulations in peritoneal cavities of CD11c^ΔIRF4 mice and controls that were Cre⁻ IRF4^fl/fl littermates or age- and sex-matched Cre⁺ mice not bearing IRF4 floxed alleles. (F and G) Quantification of F4/80⁺ macrophages and MHC II⁺ macrophages (F) and MHC II⁺ macrophage CC226⁻ or CD226⁺ subpopulations (G) in peritoneal cavities of CD11c^ΔIRF4 mice (ΔIRF4) and controls. (H and I) Quantification of F4/80⁺ ICAM2⁺ or MHC II⁺ macrophages (H) and MHC II⁺ macrophage subpopulations (I) in pleural cavities of CD11c^ΔIRF4 mice (ΔIRF4) and controls. (F–I) Data are representative of three independent experiments (n = 3–4 mice/experiment, mean ± SEM). P-values, unpaired Student’s t test: *, P < 0.05; **, P < 0.01.