Figure 2.

MyD88 deficiency has no effect on T cell development in thymus and regulatory T cell differentiation. (A) Thymocytes from 6–7-wk-old MyD88^−/−NY8.3NOD and WT NY8.3NOD mice (sex matched) were stained with fluorochrome-conjugated anti-TCRβ, anti-CD4, and anti-CD8 antibodies. n = 8–10 mice/group from two independent experiments. (B) CD8⁺ T cells in the spleen of MyD88^−/−NY8.3NOD mice. The experiment was performed in three independent experiments, and two-tailed Mann-Whitney test was used for statistical analysis. *, P < 0.05. (C) The absolute number of infiltrated CD4⁺ and CD8⁺ T cells in the islets of MyD88^−/−NY8.3NOD mice after gating with TCRβ-positive cells is shown. The data were from two independent experiments. (D) Natural regulatory T cells in MyD88^−/−NY8.3NOD mice. n ≥ 6 mice/group/experiment from two independent experiments. PLN, pancreatic LN. Data are mean ± SEM.