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. 2016 Sep 19;213(10):2187–2207. doi: 10.1084/jem.20160552

Figure 5.

Figure 5.

The inhibitory effect of the Slam locus is mediated by 2B4 with no appreciable impact from other SLAM receptors or Gm10521. (A–D) Natural cytotoxicity of IL-2–expanded NK cells from wild-type, SFR KO, 2B4 KO, SLAMF7 KO (C57BL/6 background; A), CD84 KO (C57BL/6 background; B), SLAMF6 KO (C57BL/6 background; C), or Ly-9 KO (129S1/Sv background; D) mice was analyzed toward activated CD4+ T cells from either wild-type or SFR KO mice as targets, as detailed for Fig. 3 A. Results are representative of three (A), two (B and D), or four (C) experiments. (E) Natural cytotoxicity of IL-2–expanded NK cells from wild-type mice (C57BL/6) was tested against activated CD4+ T cells from wild-type, CD48 KO, Ly-9 KO, or SLAMF6 KO mice (129S1/Sv background), as detailed for Fig. 3 A. Results are representative of four experiments. (F) Natural cytotoxicity of IL-2–expanded NK cells from wild-type mice in C57BL/6, 129S1/Sv, or DBA/1J background was determined using activated CD4+ T cells from wild-type or CD48 KO mice as targets, as detailed for Fig. 3 A. Results are representative of three experiments. (G–I) Natural cytotoxicity of IL-15–expanded NK cells from either wild-type or 2B4 KO mice was analyzed against activated CD4+ T cells from wild-type, CD84 KO, SLAMF6 KO, Ly-9 KO, CD48 KO, or SLAMF7 KO mice, as detailed for Fig. 3 A. Results are representative of four (G), three (H), or two (I) experiments. (J and K) Natural cytotoxicity of IL-2–expanded NK cells from either wild-type or Gm10521 KO mice was tested at the indicated E/T ratio toward RMA, RMA-S, YAC-1, wild-type CD4+ T cells, and SFR KO CD4+ T cells. Results are representative of two experiments. Mean values of duplicates with standard deviations are depicted.