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. Author manuscript; available in PMC: 2016 Sep 21.
Published in final edited form as: Biomaterials. 2014 Jan 15;35(10):3252–3262. doi: 10.1016/j.biomaterials.2013.12.093

Fig. 1.

Fig. 1

Composition and pro-adhesive activity of A549-CM. (A) Western blotting images of DMEM, three different batches of A549-CM and 1 μg/lane of purified ECM protein (Positive control). Antibodies used recognized: laminin (LM), fibronectin (FN), vitronectin (VT), collagen type IV (Coll IV), collagen type I (Coll I) and thrombospondin (TSP-1). (B) Adhesion of mESCs on increasing percentage of immobilized DMEM (white diamond) and A549-CM (white squares). The data are presented as the percentage of cells adhering to 1 μg/well of immobilized FN, which served as positive control. The adhesion to A549-CM was fitted using four-parameters logistic curve (R2 = 0.9928). The data are mean ± SEM of at least three independent experiments in triplicate.