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. Author manuscript; available in PMC: 2016 Sep 21.
Published in final edited form as: Biomaterials. 2014 Jan 15;35(10):3252–3262. doi: 10.1016/j.biomaterials.2013.12.093

Fig. 2.

Fig. 2

Functional expression of integrin receptors by mESCs. (A) Western blotting images of total cellular protein and positive control cells (Pos. Cont.). The corresponding specificity of each antibody used (Ab) is indicated on the left side of each image. The experiments were performed with at least three different passages of the mESCs yielding similar results; representative blots are presented. (B) Adhesion of mESCs on immobilized 1 μg/well of bovine serum albumin (BSA); collagen type IV (Coll IV); pro-collagen type I (Pro-Coll I); collagen type I (Coll I); fibronectin (FN, positive control set to 100%); laminin (LM); thrombospondin-1 (TSP-1); vascular cell adhesion molecule-1 (VCAM-1); and vitronectin (VT). The data are mean ± SEM of at least four independent experiments in triplicate. *p < 0.01 vs. BSA.