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. Author manuscript; available in PMC: 2016 Sep 21.
Published in final edited form as: Biomaterials. 2014 Jan 15;35(10):3252–3262. doi: 10.1016/j.biomaterials.2013.12.093

Fig. 4.

Fig. 4

Adsorption of CM-derived ECM proteins and mESCs repopulation of decellularized lungs. (A) Quantitation of laminin and fibronectin in CM before and after incubation with decellularized lung slices using specific ELISA. The data represent the amounts of ECM proteins in ng/mg of supernatants normalized to dry weight of decellularized lung tissue. The data are mean ± SD of three independent experiments in triplicate. *p < 0.05 after vs. before. (B) Quantitation of fluorescently-labeled mESCs retained upon repopulation in the decellularized lungs pre-treated with DMEM or CM. The fluorescent dye was extracted and the number of retained cells was determined using calibration curves of known cell numbers. The data are presented as the percentage of mESCs specifically adhered in the left lobe of the total inoculated cell number. The data are mean ± SEM of eight reseeded left lobes. *p < 0.05 CM vs. DMEM.