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. Author manuscript; available in PMC: 2016 Sep 21.
Published in final edited form as: Biomaterials. 2014 Jan 15;35(10):3252–3262. doi: 10.1016/j.biomaterials.2013.12.093

Fig. 5.

Fig. 5

Longitudinal distribution of mESCs upon inoculation into decellularized lung. (A) Schematic presentation of the rat lungs with right main bronchus ligated and mESCs inoculated via the trachea into left lobe. Dashed lines indicate the location of the sections made to generate five distinct areas along the cranial-caudal axis. (B) Fluorescently-labeled mESCs were gravitationally inoculated into DMEM (gray bars) or CM (black bars) pre-treated lobes. Following 1 h incubation the left lobes were sectioned along the dashed lines, the sections were solubilized and the cell numbers was quantitated as per the calibration curves. The data are presented as the percentage of total cell number retained in the lobe normalized to the weight of the dry tissue. The data are mean ± SEM of six reseeded left lobes. *p < 0.05 CM vs. DMEM.