Table 1.
ECM protein | Potency (EC50) | Efficacy | R2 |
---|---|---|---|
Fibronectin | 11.6 ± 0.4 | 97.8 ± 1.0% | 0.9778 |
Vitronectin | 1275.3 ± 29.0 | 84.9 ± 1.4% | 0.9943 |
Thrombospondin-1 | 607.0 ± 5.3 | 42.6 ± 1.1% | 0.9878 |
LM 511 | 72.2 ± 2.4 | 111.0 ± 1.4% | 0.9960 |
LM 521 | 77.9 ± 6.2 | 97.7 ± 1.5% | 0.9870 |
LM 332 | 177.7 ± 16.4 | 100 ± 3.2% | 0.9802 |
LM 421 | 202.6 ± 13.6 | 86.1 ± 2.0% | 0.9883 |
LM 211 | 409.7 ± 29.4 | 93.2 ± 2.9% | 0.9840 |
LM 111 | 504.4 ± 55.2 | 67.7 ± 2.0% | 0.9769 |
LM 411 | 947.9 ± 92.0 | 101.7 ± 5.5% | 0.9852 |
Potency is expressed as EC50 (ng/well) of the immobilized proteins and efficacy is expressed as the percentage cells adhered in comparison to adhesion to the positive control (1 μg/well of immobilized fibronectin). The data are mean ± SEM of three independent experiments performed in triplicates (n = 9). R2 is the correlation coefficient of the four parameters logistic curve fitted to calculate the potency and efficacy. LM –laminin.