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. Author manuscript; available in PMC: 2017 Aug 25.
Published in final edited form as: Cell. 2016 Aug 25;166(5):1324–1337.e11. doi: 10.1016/j.cell.2016.07.040

Figure 2. see also Figure S2, Table S1: Identities and concentrations of matrix metabolites in human mitochondria.

Figure 2

(A) Generation of the MITObolome and the set of 132 metabolites for which concentrations were measured. Mitochondrial proteomic data was cross-referenced with a list of all human metabolic enzymes and transporters. The overlap between these two data sets was used in conjunction with KEGG and manual curation to assemble the MITObolome, a list of all predicted metabolites within mitochondria. The MITObolome was filtered on the indicated criteria and supplemented with additional metabolites to generate the final set of 132 metabolites for which concentrations were measured. KEGG, Kyoto Encyclopedia of Genes and Genomes.

(B) Absolute quantification of matrix metabolites is highly consistent between experiments. Matrix concentrations of metabolites from two biological replicates were compared and a Pearson correlation coefficient was calculated.

(C) Concentrations of metabolites in the mitochondrial matrix and whole-cells. Data are from cells cultured in DME base media (mean ± SEM, n = 3). For each group, metabolites are arranged from most abundant to least abundant within mitochondria. Metabolites not considered to be present at levels above background are plotted as red dots on the x-axis. See Table S1 for the full names of certain abbreviated metabolites. PPP, pentose phosphate pathway.