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Yeast pol ζ extension of a control and dG-N2-IQ modified template at the G1 (top) or G3 (bottom) positions when paired by dC (left) or mispaired with dA (right). Reactions were run with 10 nM DNA, 100 μM dNTPs, and 0, 2.5, 5.0, and 10 nM of ypol ζ.
