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. 2016 Jun 17;5:1411. [Version 1] doi: 10.12688/f1000research.9022.1

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Copyright: © 2016 Attali D et al.

This is an open access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — Comparison between droplet gating in ( A) QuantaSoft and ( B) ddpcr. Both tools analyzed the same ddPCR experiment (well F05) from an assay designed to quantify wild-type (double-positive) and mutant (FAM-positive) alleles of the BRAF gene. ( A) QuantaSoft failed to assign the double-positive and FAM-positive droplets into unique clusters, instead assigning all droplets recording a high FAM signal to a single cluster; ( B) ddpcr assigned droplets into one of three uniquely identified clusters (double-positive (green), FAM-positive (orange), and empty (black)), or rain (blue).