Table 3. Primers used for the amplification of gene fragments.

Target gene	Primer name	Primer Sequence (5’ to 3’)	Reference genome	Primer coordinates	Amplicon size (bp)
Mitochondrial COI (within barcode region)	COI-AnophF	GCAGGAATTTCTTCTATTTTAGG	L20934	1,874–1,896	275
	HCO2198R 26	TAAACTTCAGGGTGACCAAAAAATCA	L20934	2,148–2,123
Mitochondrial COI	Uni-Minibar-JVF	ACAAATCATAARGATATTGGAAC	L20934	1,445–1,467	173
	Uni-Minibar-JVR	AAAATTATAATAAAWGCATGAGC	L20934	1,617–1,55
Mitochondrial COI	Mos-Co1-JVF	ATAGTWATACCTATYATAATTGG	L20934	1,622–1,644	299
	Mos-Co1-JVR	ACWGTAGTAATAAAATTTACTGC	L20934	1,920–1,898
Mitochondrial ND1	Mos-ND1F	TATGTCTTGAAAACATAAGAAAG	L20934	11,569–11,591	173
	Mos-ND1R	CGDTATGATAAATTAATGTAATTAG	L20934	11,717–11,741
Mitochondrial cyt b	CYT BF 35	GGACAAATATCATTTTGAGGAGCAACAG	L20934	10,821–10,848	470
	CYT BR 35	ATTACTCCTCCTAGCTTATTAGGAATTG	L20934	11,290–11,263
Ribosomal DNA IGS	AgamUni F 2	GTGAAGCTTGGTGCGTGCT	KT284724	126–174	169
	AgamUni R 2	GCACGCCGACAAGCTCA	KT284724	319–303

F is forward primer direction; R is reverse primer direction.