Table 2:

Toxicity of extracted chlorophyllin in laboratory condition against L. acuminata.

Exposure period (h)	Treatment	LC50 mg/L (w/v)	LCL	UCL	Slope value	t-ratio	g-value	Heterogeneity
24	Ext Chl	882.71	831.01	981.00	7.68± 1.45	5.27	0.13	0.18
48	Ext Chl	795.51	755.58	851.20	7.46± 1.35	5.52	0.12	0.27
72	Ext Chl	728.41	687.15	768.60	7.31± 1.32	5.54	0.12	0.26
96	Ext Chl	666.56	629.89	695.48	9.79± 1.44	6.75	0.08	0.48

Notes: Six batches of 10 snails were exposed to different concentrations. Mortality was determined at 24 h to 96 h. Concentrations given are the final concentration (w/v) in the glass aquarium water. Ext Chl - extracted chlorophyllin; Pure Chl - pure chlorophyllin; LCL - lower confidence limit, UCL - upper confidence limit. Significant negative regression (p<0.05) was observed between exposure time and LC₅₀ treatments. Ts - testing significance of the regression coefficient - Ext Chl −3.72⁺ to −2.23⁺ and 265.8⁺⁺ to 1268⁺⁺ was observed. ⁺Linear regression between X and Y. ⁺⁺Non-linear regression between X and Y.