Table 3:

Toxicity of chlorophyllin against L. acuminata with continuous exposure (10:00 a.m. – 02:00 p.m.) of sunlight.

Exposure period (h)	Treatment	LC50 mg/L (w/v)	LCL	UCL	Slope value	t-ratio	g-value	Heterogeneity
1	Ext Chl	938.16	715.53	1688.32	2.36±0.50	4.68	0.17	0.28
	Pure Chl	11.23	8.30	22.81	2.03±0.47	4.31	0.20	0.21
2	Ext Chl	597.05	492.40	827.10	2.18±0.43	5.08	0.14	0.15
	Pure Chl	7.43	5.85	11.76	1.75±0.39	4.40	0.19	0.22
3	Ext Chl	492.68	408.85	645.86	2.04±0.41	4.94	0.15	0.25
	Pure Chl	4.17	3.38	5.04	2.06±0.38	5.38	0.13	0.31
4	Ext Chl	331.01	287.98	374.68	3.21±0.44	7.22	0.07	0.48
	Pure Chl	2.60	1.96	3.13	2.43±0.40	6.03	0.10	0.59

Notes: Six batches of 10 snails were exposed to different concentrations. Mortality was determined at 1 h up to 4 h. Concentrations given are the final concentration (w/v) in the glass aquarium water. Ext Chl - extracted chlorophyllin, Pure Chl - pure chlorophyllin, LCL - lower confidence limit, UCL - upper confidence limit. Significant negative regression (p<0.05) was observed between exposure time and LC₅₀ of treatments. Ts - testing significant of the regression coefficient - Ext Chl −343.9⁺ to −41.22⁺ and 0.0⁺⁺ to 1973⁺⁺ and Pure Chl −4.47⁺ to −1.35⁺ and 0.0⁺⁺ to 27.00⁺⁺ was observed. ⁺Linear regression between X and Y. ⁺⁺Non-linear regression between X and Y.