Table 4:

Toxicity of chlorophyllin against L. acuminata with discontinuous (1 h interval) exposure in sunlight.

Exposure period (h)	Treatment	LC50 mg/L (w/v)	LCL	UCL	Slope value	t-ratio	g-value	Heterogeneity
2	Ext Chl	686.57	562.90	1098.42	4.12± 0.93	4.42	0.19	0.94
	Pure Chl	12.19	9.51	22.06	2.64± 0.61	4.30	0.20	0.25
4	Ext Chl	556.74	481.10	733.07	3.90± 0.74	5.26	0.13	0.67
	Pure Chl	8.83	7.30	12.87	2.32± 0.51	4.53	0.18	0.18
6	Ext Chl	451.68	403.14	538.18	4.24± 0.67	6.27	0.11	0.79
	Pure Chl	6.50	5.60	7.86	2.56± 0.49	5.21	0.14	0.31
8	Ext Chl	357.04	320.26	403.29	4.74± 0.6	7.39	0.11	0.58
	Pure Chl	4.94	4.41	5.46	4.02± 0.52	7.61	0.06	0.73

Notes: Six batches of 10 snails were exposed to different concentrations. Mortality was determined at 2 h to 8 h each with 1 h intervals. Concentrations given are the final concentration (w/v) in the glass aquarium water. Ext Chl - extracted chlorophyllin, Pure Chl - pure chlorophyllin, LCL - lower confidence limit, UCL - upper confidence limit. Significant negative regression (p<0.05) was observed between exposure time and LC₅₀ treatments. Ts - testing significant of the regression coefficient - Ext Chl −66.85⁺ to −42.51⁺ and 0.0⁺⁺ to 1268⁺⁺ and Pure Chl −1.81⁺ to −0.59⁺ and 0.0⁺⁺ to 25.10⁺⁺ was observed. ⁺Linear regression between X and Y. ⁺⁺Non-linear regression between X and Y.