Table 5:

Toxicity of chlorophyllin in the presence of different spectra of light against L. acuminata.

Exposure period (h)	Treatment	Different spectra of light	LC50 mg/L (w/v)	LCL	UCL	Slope value	t-ratio	g-value	Heterogeneity
24	Ext Chl	Green	1428.47	1060.01	3264.72	2.71±0.69	3.91	0.25	0.37
		Violet	1427.16	1051.04	3338.91	2.55±0.65	3.89	0.25	0.32
		Blue	1090.88	881.95	1721.74	2.72±0.58	4.63	0.17	0.20
		Orange	1009.97	819.65	1567.96	2.47±0.54	4.57	0.18	0.29
		Red	905.52	744.63	1341.32	2.32±0.51	4.51	0.18	0.22
		White	708.74	609.36	883.19	2.55±0.49	5.11	0.14	0.17
		Yellow	619.73	548.31	711.90	3.19±0.51	6.25	0.09	0.23
48	Ext Chl	Green	1273.84	920.19	3510.62	1.86±0.53	3.51	0.31	0.20
		Violet	1328.08	953.03	3682.09	1.92±0.53	3.56	0.30	0.23
		Blue	864.70	712.37	1269.62	2.23±0.50	4.41	0.19	0.14
		Orange	775.75	663.90	997.24	2.61±0.51	5.12	0.14	0.43
		Red	721.37	620.75	901.36	2.59±0.50	5.17	0.14	0.29
		White	574.21	492.87	673.09	2.62±0.48	5.38	0.13	0.25
		Yellow	533.09	465.93	604.48	3.14±0.49	6.30	0.09	0.35
72	Ext Chl	Green	1148.51	831.01	3488.41	1.61±0.49	3.23	0.36	0.12
		Violet	1017.82	784.95	2018.16	1.86±0.50	3.72	0.27	0.22
		Blue	764.53	649.07	1000.67	2.43±0.50	4.86	0.16	0.20
		Orange	630.78	541.77	761.17	2.51±0.48	5.15	0.14	0.37
		Red	556.12	470.96	655.76	2.47±0.48	5.13	0.14	0.31
		White	494.64	418.94	568.22	2.77±0.48	5.69	0.11	0.32
		Yellow	468.70	408.55	524.83	3.50±0.50	6.88	0.08	0.41
96	Ext Chl	Green	833.02	633.91	1927.15	1.41±0.47	3.01	0.42	0.13
		Violet	826.81	670.98	1278.36	1.95±0.48	4.01	0.23	0.30
		Blue	621.33	527.88	758.46	2.35±0.48	4.87	0.16	0.31
		Orange	505.02	421.30	588.74	2.50±0.48	5.21	0.14	0.37
		Red	472.42	403.63	536.33	3.05±0.49	6.17	0.10	0.33
		White	432.66	368.54	488.57	3.32±0.50	6.53	0.09	0.54
		Yellow	392.77	342.63	436.07	4.28±0.56	7.60	0.06	0.60

Notes: Six batches of 10 snails were exposed to different concentrations. Mortality was determined at every 24 h up to 96 h. Concentrations given are the final concentration (w/v) in the glass aquarium water. Ext Chl - extracted chlorophyllin, LCL - lower confidence limit, UCL - upper confidence limit. Significant negative regression (p<0.05) was observed between exposure time and LC₅₀ of treatments. Ts - testing significant of the regression coefficient of green light −13.31⁺ to −2.62⁺ and 0.0⁺⁺ to 2520⁺⁺, violet light −13.73⁺ to −3.86⁺ and 0.0⁺⁺ to 2629⁺⁺, blue light −9.46⁺ to −3.10⁺ and 0.0⁺⁺ to 1889⁺⁺, orange light −10.12⁺ to −3.71⁺ and 0.0⁺⁺ to 1887⁺⁺, red light −9.05⁺ to −3.14⁺ and 0.0⁺⁺ to 1685⁺⁺, white light −5.89⁺ to −1.67⁺ and 0.0⁺⁺ to 1188⁺⁺, yellow light −3.63⁺ to −2.58⁺ and 0.0⁺⁺ to 1018⁺⁺ was observed. ⁺Linear regression between X and Y. ⁺⁺Non-linear regression between X and Y.