Figure 3.

Gel map of protein spots changing significantly and identified. This 2-D gel is a representative image of the Commassie Brillant Blue R-250 (CBB) stained gel from (A) embryos or (B) endosperms of Yliangyou 2 hybrid rice seeds aged in 100% relative humidity and at 40°C for 10 days. A total of 450 μg of proteins from embryos or endosperms of seeds aged for different periods of time was extracted and separated by 2-D gel as described in Materials and methods, and visualized with CBB. The numbered protein spots changed differentially are indicated by arrows and described in Tables 1, 2.