Table 3.
The proteins changing in abundance and identified in embryo and endosperm during aging of Yliangyou 2 hybrid rice seeds.
| Biological process | Embryo | Endosperm | ||||||
|---|---|---|---|---|---|---|---|---|
| Increased | Decreased | Increased | Decreased | |||||
| No. | Spot ID | No. | Spot ID | No. | Spot ID | No. | Spot ID | |
| Metabolism | 5 | 29 | ||||||
| Amino acid | 4 | 10, 11, 34, 40 | 11 | 18′, 19′, 35′, 38′, 39′, 47′, 48′, 52′, 56′, 57′, 61′ | ||||
| Sugar and polysaccharide | 1 | 52 | 16 | 1′, 2′, 3′, 4′, 5′, 6′, 7′, 8′, 9′, 15′, 16′, 17′, 42′, 44′, 64′, 65′, | ||||
| Nucleotide | 1 | 53 ′ | ||||||
| Lipid | 1 | 31′ | ||||||
| Energy | 19 | 1 | 21 | |||||
| Glycolysis | 7 | 13, 24, 32, 47, 49, 65, 66 | 1 | 41 | 7 | 23′, 37′, 68′, 69′, 70′, 71′, 75′ | ||
| TCA cycle | 1 | 14 | 3 | 26′, 54′, 59′, | ||||
| Respiration | 2 | 35, 36 | 1 | 43′ | ||||
| Fermentation | 5 | 16, 17, 18, 20, 42 | 4 | 28′, 29′, 30′, 55′ | ||||
| Glyoxylate cycle | 3 | 4, 5, 48 | 2 | 73′, 74′ | ||||
| Photosynthesis | 1 | 33 | 3 | 10′, 11′, 12′ | ||||
| Others | 1 | 60′ | ||||||
| Cell growth and division | 1 | 79 | 1 | 100′ | 3 | 25′, 51′, 97′ | ||
| Transcription | 2 | 46, 63 | 1 | 81 | ||||
| Protein synthesis and destination | 5 | 8 | ||||||
| Protein synthesis | 4 | 6, 7, 19, 39 | 4 | 13′, 32′, 49′, 93′ | ||||
| Protein folding | 1 | 36′ | ||||||
| Proteolysis | 1 | 67 | 2 | 40′, 83′ | ||||
| Protein transport | 1 | 50′ | ||||||
| Storage protein | 11 | 29, 30, 38, 51, 53, 56, 57, 58, 64, 71, 75 | 5 | 20′, 77′, 79′, 95′, 96′ | ||||
| Cell defense and rescue | 15 | 1 | 7 | |||||
| Defense-related | 1 | 69 | 1 | 62′ | ||||
| Detoxification | 6 | 31, 54, 55, 60, 68, 82 | 2 | 84′, 85′ | ||||
| Stress response | 8 | 1, 2, 12, 27, 28, 43, 44, 86 | 1 | 76 | 4 | 21′, 22′, 24′, 86′ | ||
| Unknown | 3 | 63′, 76′, 82′ | ||||||
| Total | 58 | 3 | 1 | 76 | ||||
Only protein spots which changed in volume at least 2-fold (P < 0.05) in all three replicates for a given treatment are included. The positions of the protein spots are shown in Figure 3. The bold values represent the protein spot number of functional group.