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. 2016 Sep 13;22:3248–3750. doi: 10.12659/MSM.899980

Figure 2.

Figure 2

pRC/CMV-mediated upregulation and siRNA-mediated knockdown of Sam68 in ovarian cancer OVCAR-3 cells (A) mRNA level of Sam68 in OVCAR-3 cells, which were transfected with pRC/CMV-Sam68-FLAG (Sam68 +) or pcRC/CMV (Con +, as control); (B) Western blot analysis of Sam68 in the blank, Sam68 +, or Con + OVCAR-3 cells; (C) mRNA level of Sam68 in the OVCAR-3 cells, which were transfected with siRNA-Con or siRNA-Sam68 (20 or 40 nM); (D) Western blot analysis of Sam68 in the siRNA-Con- or siRNA-Sam68-transfected (20 or 40 nM) OVCAR-3 cells. Data was averaged for triple independent results. ns represented no significance, ** represented p<0.01, and *** represented p<0.001.