Figure 2.

MLC2 is hyper-phosphorylated in spasm provocation tests. Provocation of coronary artery spasm was performed with an intracoronary injection of 5-HT in rats. (A) IHC analysis of the p-MLC2 in the coronary artery at the PBS-injected segment. (B) IHC staining of p-MLC2 in the spastic coronary segment in the 5-HT-injected group of rats. (C) In the vehicle (PBS)-treated heart, the interstitial small artery adjacent to the injection site was stained with a specific antibody against p-MLC2 using IHC analysis. (D) In the 5-HT-treated coronary arteries, the interstitial small artery which was adjacent to the injection site was stained with p-MLC2 antibody using IHC. Red arrows highlight the positive staining of p-MLC2. Magnification: 400×.