Characterization of ASCs. (A): The cultured ASCs (n = 3 experiments) were positive for CD29, CD59, and CD90.1 and negative for CD11b and CD45. (B): The ASCs appeared spindle-shaped under phase-contrast microscopy. (C): Fluorescence microscopy images of ASCs expressed strong blue GFP fluorescence. (D): Prussian blue staining showed blue magnetic SPIO particles distributed around the nuclei of SPIOASCs. Scale bar = 50 μm. Abbreviations: ASC, adipose-derived stem cell; FITC, fluorescein isothiocyanate; GFP, green fluorescent protein; SPIO, superparamagnetic iron oxide; SPIOASC, superparamagnetic iron oxide-labeled adipose-derived stem cell.