Figure 1.

Diagram representing the feeder-free and serum-free erythroid differentiation of hPSCs augmented by the addition of small molecules. Abbreviations: BMP, bone morphogenic protein; EBs, embryoid bodies; EPO, erythropoietin; FGF, fibroblast growth factor; Flt3L, Flt3-ligand; hPSCs, human pluripotent stem cells; HSPCs, hematopoietic stem and progenitor cells; IBIT, IMDM + bovine serum albumin, insulin, transferrin; IBMX, isobutyl methyl xanthine; IGF, insulin-like growth factor; IL, interleukin; RBCs, red blood cells; SCF, stem cell factor; TPO, thrombopoietin; VEGF, vascular endothelial growth factor 165.