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. 2016 Jul 8;5(10):1394–1405. doi: 10.5966/sctm.2015-0371

Figure 6.

Figure 6.

High-performance liquid chromatography (HPLC) analysis of the globin chains expressed at day 31 of H1 human embryonic stem cell (hESC) differentiation. (A): Example of elution profiles obtained by HPLC of H1 hESC-derived erythroid cells with (SM) and without (DMSO) small molecules and of the adult and fetal control used to determine the elution time of the different globin chains. (B): Globin chains were assessed at the protein level after erythroid differentiation with (SM) or without (DMSO) the 4 small molecules added (inhibitor VIII + isobutyl methyl xanthine + SR1 + SC1). Lysates from adult peripheral blood and fetal liver red blood cells were used as reference controls to establish the elution time of each peak. Abbreviations: DMSO, dimethyl sulfoxide; SM, small molecules.