NAFLD is associated with peroxisomal dysfunction. The docosahexaenoic acid (DHA, 22:6n3) to docosapentaenoic acid (DPA, 22: 5n3) ratio among different lipid classes and changes in plasmalogen levels are represented graphically. There is a generalized trend for decrease in DHA:DPA ratio, which was significant for phosphatidylcholine (PC) and phosphatidylethanolamine (PE) pools (A). Similarly, there was a stepwise decrease in plasmalogen levels (nmol/g of sample) from normal to NAFL to NASH that was statistically significant for total plasmalogen (dm), dm 16:0, and dm 18:1n9 content in NASH only (B). The plasmalogen concentration within lipid classes (mol%) was significantly decreased for dm and dm16:0 within PC pool in NAFLD (C). These figures suggest that NAFLD is associated with peroxisomal dysfunction, which is most marked in NASH. Data expressed as means ± SEM. *P < 0.05; **P < 0.01; ***P < 0.001 versus normal.