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. 2016 Sep 21;14:81. doi: 10.1186/s12915-016-0303-0

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© Stojanoski et al. 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — Immunoblot indicating expression levels and localization of N-terminal His-tagged, full-length wild-type MCR-1 (lane 1–4) and T285A (lane 5–8) mutant. L whole cell lysis, P pellet, S soluble, D detergent-soluble membrane fraction. Lane 9 (+) purified His-MCR-1