Fig. 3.

NT1014 induced cell cycle G1 arrest and cellular apoptosis. The IGROV-1 and SKOV3 cell lines were treated with NT1014 for 24 h. Cell cycle progression was analyzed by Cellometer. NT1014 induced G0/G1 cell cycle arrest and reduced S phase in a dose-dependent manner in both cell lines (a, b). The effect of NT1014 on cell cycle-related proteins (p21, p27, cyclin D1, CDK4, and CDK6) was assessed by Western blotting (c). Both cells were treated with varying doses of NT1014 for 24 h, and cell apoptosis was examined by an annexin V FITC assay via Cellometer. NT1014 significantly increased annexin V expression in a dose-dependent manner (d). The activity of cleaved caspase 3 was detected by ELISA assay after treatment of NT1014 for 4 h (e). Western blotting showed that NT1014 decreased the expression of BCL-xL and Mcl-1 in the IGROV-1 and SKOV3 cell lines after exposure to NT1014 for 24 h (f). α-tubulin used as a loading control. *p < 0.05 and **p < 0.01