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. 2016 Sep 21;8:101. doi: 10.1186/s13148-016-0269-3

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — The MSP-HTPrimer sequence: the principle of in silico deamination workflow. The MSP-HTPrimer pipeline takes into account that deamination of double-stranded native DNA (left) results in four different template sequences (right) for primer design. Thus, from the plus and minus strand, different methylation-dependent primer pairs can be designed (M - methylated; U - unmethylated; Cs in blue - not in a CpG context are usually unmethylated)