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. Author manuscript; available in PMC: 2017 Aug 15.
Published in final edited form as: J Immunol. 2016 Jul 13;197(4):1089–1099. doi: 10.4049/jimmunol.1501798

FIGURE 2.

FIGURE 2

Expression of CXCR3 ligands and their common receptor in AA lesional skin. (A) Detection of CD4, CD8, and CXCR3 expression on HF infiltrating cells in human AA scalp and normal control by immunohistochemistry. Double immunofluorescent staining of CXCR3 (green) and CD3 (red) is also shown. (B) Immunofluorescent staining of CXCL9 (red), CXCL10 (red) and CXCL1 (red) expression in human AA scalp and normal control. (C) Double immunofluorescent staining of CD8 (green) and CXCR3 (red) on HF infiltrating cells in skin sections from C3H/HeJ AA and non-alopecia C3H/HeJ mice. (D) Detection of CXCL9, CXCL10, and CXCL11 expression in skin sections from C3H/HeJ AA and non-alopecia C3H/HeJ mice by immunohistochemistry where CXCL9, CXCL10, and CXCL11 expression is indicated by the presence of red precipitants. Data are representative of four separate samples in each staining. Scale bar = 200μM.