Skip to main content
. Author manuscript; available in PMC: 2017 Aug 15.
Published in final edited form as: J Immunol. 2016 Jul 13;197(4):1089–1099. doi: 10.4049/jimmunol.1501798

FIGURE 3.

FIGURE 3

IFN-γ upregulates CXCR3 ligand expression in HF. (A) Normal human anagen HFs were microdissected and cultured in vitro for 48 hours in the presence of 50 ng/ml rhIFN-γ and 25 ng/ml rhTNF-α or 50 ng/ml rhIFN-γ alone (B). Upregulation of CXCL9, CXCL10, and CXCL11 in HF was determined by immunohistochemistry. Relative expression of CXCL9, CXCL10, and CXCL11 genes in normal human HF was determined by quantitative RT-PCR. Quantitative RT-PCR results were normalized to GAPDH and represent mean ± SEM relative expression from PBS and IFN-γ and TNF-α treatment. Data are representative of four independent experiments. Scale bar = 200μM. * indicates p <0.05, ** indicates p<0.01.