Table 1. Primers used in PCR with the restriction sites shown as underlined.

Primers		Sequence	Restriction sites	Annealing temperature
HSPX	F1	GAGGCATCATATGGCCACCAC	NdeI	58
	R1	ACGGACCCAGTGGTCAGTTGG	None	62
	R2	GTAGGATCCGTTGGTGGACCGGAT	BamHI	57
PE35	F2	CATATGGAAAAAATGTCACATGATCCG	NdeI	64
	F3	GGTGGATCCATGGAAAAAATGTCACA	BamHI	64
	R3	GTGATCACTCCCTCCGATGTGTTGG	None	69
	R4	AACAAGCTTGATCACTCCCTCCGATGT	HindIII	68
tnPstS1a	F4	CATATGAAAATTCGTTTGCATACGCTGTTGG	NdeI	68
	F5	GTAGGATCCGCCGGGACGGTCAACATTG	BamHI	61
	R5	TATAAGCTTCTACGCGGGCGGCAGCGGCTG	HindIII	68
FbpC1b	F6	TAACATATGGCCCCATACGAGAACCTG	NdeI	68
	F7	TAAGGATCCGCCCCATACGAGAACCTG	BamHI	71
	R6	GCTCGCATCGGCACCTGGCTTAG	None	65
	R7	CACCAAGCTTAGCGGATCGCACCGACG	HindIII	74

As reported previously

^a[28],

^b[29]