Figure 2.

Habituation of the C-start in zebrafish larvae can persist for ≥ 18 h. (A) Experimental protocol for the fish that received habituation training. Here, posttests occurred at 1 h and 18 h after training. The Test Alone group did not receive habituation training but was otherwise treated identically to the Trained group. After the 1 h Posttest, larvae were left in the dark, unstimulated, for 15 h. Then the room lights were turned on for 1 h after which there was a second posttest. (B) LTH of the C-start. The trained larvae exhibited significantly greater habituation at both the 1 h and 18 h Posttests than did the larvae given the test alone treatment (asterisks).