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. 2016 Mar 21;35(39):5155–5169. doi: 10.1038/onc.2016.47

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Copyright © 2016 Macmillan Publishers Limited

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Increased Lpd expression correlates with poor prognosis for breast cancer patients. (a) Western blot analysis of Lpd expression in human breast cancer cell lines with varying metastatic potential. Loading control: Tubulin. (b) Kaplan–Meier analysis of metastasis-free survival in the NKI295 data set. Patients were stratified by expression of Lpd. The P-value was calculated by a log-rank test. (c) Kaplan–Meier analysis of disease-free survival in the NKI295 data set. Patients were stratified by expression of Lpd. The P-value was calculated by a log-rank test. (d) Kaplan–Meier plots of breast cancer-associated mortality of histoscores 1–3 for Lpd intensity at the plasma membrane. Histoscore 2: HR (95% CI): 2.23 (1.26–3.95). (e) Representative examples of Lpd immunohistochemistry staining for histoscores 1–3 for Lpd staining intensity at the plasma membrane. Scale bar, 5 μm. See also Supplementary Figures S1 and S2.