Skip to main content
. Author manuscript; available in PMC: 2017 Oct 1.
Published in final edited form as: Dev Biol. 2016 Aug 6;418(1):204–215. doi: 10.1016/j.ydbio.2016.08.005

Fig. 2.

Fig. 2

Double knockout mutations of Ncor1 and Ncor2 generated using CRISPR/Cas9 gene editing result in ectopic Fgf8 expression and FGF signaling in both heart and caudal domains. (A–B) Shown is expression of Fgf8 (panel A) or the FGF target gene Spry2 (panel B) for wild-type (WT) or CRISPR mutants; arrows mark the posterior border of the heart expression domain and the anterior border of the caudal expression domain; h, heart (mutants exhibit distended heart tube); the caudal end of embryo #12 was cut off in order to align with a WT embryo; embryo #3 lateral view (left panels) has the heads aligned with WT to best compare the heart domains, whereas the dorsal view (middle panels) has the tails aligned with WT to best compare the caudal domains. (C) DNA sequences of the three embryos shown above demonstrate biallelic insertion or deletion mutations in both Ncor1 and Ncor2 that are expected to be null mutations.