Table 1.
Embryos obtained from CRISPR/Cas9 gene editing of Ncor1/Ncor2.
| Embryo | Number of alleles with deletions/ Genotype |
Somites (Uncx) |
Heart Tube | FGF signaling (Fgf8 or Spry2) |
|---|---|---|---|---|
| 10 | (0) Ncor1+/+;Ncor2+/+ | normal | normal | # |
| 2 | (2) Ncor1+/−;Ncor2+/− | normal | normal | normal Fgf8 |
| 7 | (2) Ncor1+/−;Ncor2+/− | normal | normal | # |
| 8 | (2) Ncor1+/−;Ncor2+/− | normal | normal | # |
| 4 | (2) Ncor1+/−;Ncor2+/− | normal | normal | normal Fgf8 |
| *19 | (3) Ncor1−/−;Ncor2+/− | small | distended | # |
| *20 | (3) Ncor1−/−;Ncor2+/− | small | distended | # |
| *5 | (3) Ncor1+/−;Ncor2−/− | small | distended | # |
| *6 | (3) Ncor1+/−;Ncor2−/− | small | distended | # |
| *11 | (3) Ncor1−/−;Ncor2+/− | # | distended | Ectopic Spry2 |
| *16 | (3) Ncor1−/+;Ncor2−/− | small | distended | # |
| *12 | (4) Ncor1−/−;Ncor2−/− | # | distended | Ectopic Fgf8 |
| *14 | (4) Ncor1−/−;Ncor2−/− | small | distended | # |
| *3 | (4) Ncor1−/−;Ncor2−/− | # | distended | Ectopic Spry2 |
| *23 | (4) Ncor1−/−;Ncor2−/− | small | distended | # |
| *9 | (4) Ncor1−/−;Ncor2−/− | small | distended | # |
| *13 | (4) Ncor1−/−;Ncor2−/− | # | distended | Ectopic Spry2 |
| *17 | (4) Ncor1−/−;Ncor2−/− | # | distended | Ectopic Fgf8 |
Total 24 embryos with 6 embryos (numbers 1, 15, 18, 21, 22, 24) not examined as they were mosaic for DNA sequence near exon 1 for Ncor1 and/or Ncor2 (more than two alleles were obtained often including wild-type sequence).
*
, embryos with mutant phenotype;
#
, not analyzed.