Table 2.

Embryos obtained from CRISPR/Cas9 gene editing of Fgf8 RARE.

Embryo	bp deleted from RARE for each allele	Somites (Uncx)	Heart Tube	Fgf8 expression
1	0/0	#	normal	normal
2	0/0	normal	normal	#
7	0/0	normal	normal	#
14	0/0	normal	normal	#
28	0/0	normal	normal	#
30	0/0	normal	normal	normal
34	0/0	normal	normal	#
16	0/1	normal	normal	normal
17	0/0	normal	normal	#
24	0/0	normal	normal	normal
49	0/0	normal	normal	#
10	0/2	normal	normal	#
18	1/1	normal	normal	#
25	0/2	normal	normal	normal
45	0/1	normal	normal	#
12	0/3	normal	normal	#
60	0/3	#	normal	normal
48	1/2	normal	normal	#
11	2/2	normal	normal	#
42	2/2	normal	normal	normal
43	2/2	normal	normal	#
20	0/6	normal	normal	normal
33	0/6	normal	normal	#
40	0/6	normal	normal	normal
47	0/6	normal	normal	#
70	0/6	#	normal	normal
23	0/7	normal	normal	normal
29	0/8	normal	normal	normal
31	0/9	normal	normal	normal
19	0/10	#		normal
50	0/10	normal	normal	#
21	0/12	normal	normal	normal
26	0/12	normal	normal	normal
58	0/8	#		normal
5	1/11	normal	normal	#
*65	2/4	#		ectopic
55	2/5	normal	normal	normal
*38	2/6	small	normal	#
*22	2/6	small	normal	ectopic
41	2/6	normal	normal	normal
32	4/6	normal	normal	#
64	5/5	normal	normal	#
*3	6/6	small	normal	ectopic
*4	6/6	small	normal	#
*6	6/6	small	normal	#
*9	5/7	small	normal	#
44	6/6	normal	normal	#
46	6/6	normal	normal	#
54	6/7	normal	normal	#
*27	6/12	small	normal	ectopic
61	6/12	#	normal	normal
*63	6/12	small	normal	#
52	12/12	normal	normal	#
56	12/12	#	normal	normal

Total 70 embryos with 16 embryos (numbers 8, 13, 15, 35, 36, 37, 39, 51, 53, 57, 59, 62, 66, 67, 68, 69) not examined as they were mosaic for DNA sequence near the Fgf8 RARE (more than two alleles were obtained often including wild-type sequence.

^*, embryos with mutant phenotype;

^†, too young for determination;

^#, not analyzed.