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. Author manuscript; available in PMC: 2017 Oct 1.
Published in final edited form as: Dev Biol. 2016 Jul 14;418(1):146–156. doi: 10.1016/j.ydbio.2016.07.007

Figure 4.

Figure 4

Mutations of the zinc finger domains did not affect the expression of Nanos 2 in the small micromeres. Synthetic mRNAs containing the GFP ORF alone (A), or fused in frame with the Nanos 2 ORF (B,C,D) were injected in Sp fertilized eggs. In each case, the ORF was surrounded by Nanos 2 5′ and ΔGNARLE 3′UTRs. The wild type Nanos 2 was used as a control (B). The last cysteine of the first zinc finger domain (C), or the second zinc finger domain (D) present in Nanos 2 protein were mutated into a serine. Each GFP Nanos 2 RNA was co-injected with a mCherry mRNA containing the Xenopus β-globin 5′ and 3′UTRs (A’ to D’). GFP and mCherry fluorescence were assayed 18 hours post-fertilization at mesenchyme blastula. A and A’ represent the same embryo, B and B’ represent another one, etc. For GFP images, A to D were obtained using the same settings (laser intensity, pin-hole opening). For mCherry images, A’ to D’ were also taken using the same settings.