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. Author manuscript; available in PMC: 2017 Oct 1.
Published in final edited form as: Dev Biol. 2016 Jul 14;418(1):146–156. doi: 10.1016/j.ydbio.2016.07.007

Figure 6.

Figure 6

Nanos 2 N terminal domain contains an element that causes its degradation at the blastula stage. Synthetic mRNAs containing the GFP ORF alone (D,H) or fused with the Nanos 2 full length ORF (A,E) were injected in Sp fertilized eggs. Deletion mutants were also injected: GFP fused to the NPDE (N term 4: B,F) and GFP fused to Nanos 2 ORF deleted in the NPDE (N term 5:C,G). In each case, the ORF was surrounded by Nanos 2 5′ and ΔGNARLE 3′UTRs. A control RNA containing mCherry flanked with β-globin UTRs was co injected with each deletion construct. Images were taken at mesenchyme blastula stage. The GFP and mCherry fluorescence were imaged using respectively the same settings: GFP (A to F), and mCherry (A’ to F’) at 400x magnification.