Figure 3.

Single-Molecule Transcription by RP_ITC≤7 Complexes

Frame time: 200 ms.

(A) Time trace showing an increase to a stable E^∗∼0.37 state.

(B) Time trace showing pausing at E^∗∼0.37 (highlighted blue), followed by an excursion to the E^∗∼0.45 state (highlighted yellow). The return to the stable E^∗∼0.37 is assigned to RNA backtracking.

(C) Time trace showing pausing, followed by an excursion to E^∗∼0.45 (as in B), followed by a return to the RP_o baseline (assigned to RNA release).

(D) Dwell-time histograms and exponential fits for the paused state (left; n = 84) and the E^∗∼0.45 state (right; n = 60).

(E) Time trace showing no pausing before reaching E^∗∼0.45, followed by a return (highlighted yellow) to a stable E^∗∼0.37 state.