Figure 6.

PPP Perturbation Disrupts Circadian Oscillations in Mouse Tissues

(A and B) Bioluminescence recordings of suprachiasmatic nuclei (SCN) (A) and liver slices (B) from mPer2^Luciferase (mPer2^Luc) mice treated with the indicated concentration of 6AN or control (DMSO) (left), followed by wash off (right) with control medium.

(C and D) Quantifications of the period length from SCN (C) and liver (D) slices treated with 6AN or control (DMSO) before and after wash off (mean ± SEM, n = 3–7; two-tailed Student’s t test; 500 μM versus control; ^∗∗∗p < 0.001, ^∗∗p < 0.01). NS, not statistically significant by t test.

(E) Single-cell bioluminescence traces of SCN slices from mPer2^Luc mice incubated either with 500 μM 6AN or control (DMSO) (n > 100).

(F) Violin plot representing the difference in the distribution of circadian period lengths in the 6AN and control (DMSO) conditions (n > 100; two-tailed Student’s t test, p < 1 × 10⁻¹⁶).