Fig. 1.

BCL-2 is up-regulated and confers resistance to cisplatin in OTSCC cells. a mRNA and b protein levels of BCL-2 in normal tongue epithelial cell (NTEC) and OTSCC cell lines. c Protein levels of Bcl-2 after Bcl-2 transfection (pEMD-Bcl2) in NTEC cells. d Overexpression of Bcl-2 promotes NTEC growth. Cells are electroporated with 1.5 μg pEMD, or pEMD-Bcl2 and cultured for 48 h prior to WB and monitoring growth. Different inhibitory effects of cisplatin on the proliferation (e) and survival (f) in SCC-25, SCC-9, CAL27 and Tca3188 cells. Cisplatin at 5 µM was used. Data were presented as mean ± SD. *p < 0.05